Sinorhizobium meliloti regulator MucR couples exopolysaccharide synthesis and motility.

TitleSinorhizobium meliloti regulator MucR couples exopolysaccharide synthesis and motility.
Publication TypeJournal Article
Year of Publication2008
AuthorsBahlawane, C, McIntosh, M, Krol, E, Becker, A
JournalMol Plant Microbe Interact
Volume21
Issue11
Pagination1498-509
Date Published2008 Nov
ISSN0894-0282
KeywordsBacterial Physiological Phenomena, Bacterial Proteins, Base Sequence, Electrophoretic Mobility Shift Assay, Gene Expression Regulation, Bacterial, Models, Biological, Molecular Sequence Data, Oligonucleotide Array Sequence Analysis, Polysaccharides, Bacterial, Quorum Sensing, Reverse Transcriptase Polymerase Chain Reaction, Sequence Homology, Nucleic Acid, Signal Transduction, Sinorhizobium meliloti, Transcription Factors
Abstract

In order to enter symbiosis with its legume partner, Sinorhizobium meliloti requires regulatory systems for the appropriate responses to its environment. For example, motility is required for the chemotactic movement of bacteria toward the compounds released by its host, and exopolysaccharides (EPS) are required for bacterial attachment to the root or for invasion of the infection thread. Previous research has shown that ExoR/ExoS/ChvI as well as the ExpR/Sin quorum-sensing system inversely regulate both motility and EPS production, although the regulation mechanisms were unknown. We were able to attribute the ExpR-mediated regulation of motility to the ability of ExpR to bind a DNA sequence upstream of visN when activated by N-acyl-homoserine lactone. Furthermore, MucR, previously characterized as a regulator of EPS production, also affected motility. MucR inhibited expression of rem encoding an activator of motility gene expression and, consequently, the expression of Rem-regulated genes such as flaF and flgG. Binding of MucR to the rem promoter region was demonstrated and a sequence motif similar to the previously identified MucR binding consensus was identified within this region. The swarming ability of S. meliloti Rm2011 was shown to depend on a functional ExpR/Sin quorum-sensing system and the production of both flagella and EPS. Finally, we propose a model for the coordination of motility and EPS synthesis in S. meliloti.

DOI10.1094/MPMI-21-11-1498
Alternate JournalMol. Plant Microbe Interact.