TLM-Tracker: Software for cell segmentation, tracking and lineage analysis in time-lapse microscopy movies.

TitleTLM-Tracker: Software for cell segmentation, tracking and lineage analysis in time-lapse microscopy movies.
Publication TypeJournal Article
Year of Publication2012
AuthorsKlein, J, Leupold, S, Biegler, I, Biedendieck, R, Münch, R, Jahn, D
JournalBioinformatics
Volume28
Issue17
Start Page2276
Pagination2276-2277
Date Published2012 Jul 5
ISSN1367-4811
Abstract

MOTIVATION: Time-lapse imaging in combination with fluorescence microscopy techniques enable the investigation of gene regulatory circuits and uncovered phenomena like culture heterogeneity. In this context computational image processing for the analysis of single cell behaviour plays an increasing role in systems biology and mathematical modeling approaches. Consequently, we developed a software package with graphical user interface for the analysis of single bacterial cell behaviour. RESULTS: A new software called TLM-Tracker allows for the flexible and user friendly interpretation for the segmentation, tracking and lineage analysis of microbial cells in time-lapse movies. AVAILABILITY: The software package, including manual, tutorial video and examples, is available as Matlab code or executable binaries at http://www.tlmtracker.tu-bs.de. CONTACT: r.muench@tu-bs.de.

DOI10.1093/bioinformatics/bts424